ERIK D. ANDRULIS
Email:
ikandrulis@yahoo.com
EDUCATION
1993-1998 Ph.D.,
Biochemistry and Molecular Biology, Stony Brook University
1988-1992
B.S., Biological Sciences: Molecular
Genetics, University of Rochester
PROFESSIONAL APPOINTMENTS
2003-2015 Assistant Professor, Department
of Molecular Biology and Microbiology, Case Western Reserve University School
of Medicine
1998-2003 Postdoctoral Fellow,
Department of Molecular Biology and Genetics, Cornell University
1992-1993
Predoctoral Fellow,
National Cancer Institute, National Institutes of Health
MEMBERSHIPS IN
PROFESSIONAL SOCIETIES
1997-2014 Member, American Society for
Biochemistry and Molecular Biology
HONORS AND AWARDS
2012 2nd place, CWRU Martin Luther King, Jr.
Faculty/Staff Essay Contest
2003-2007 Mount Sinai Health Care
Foundation Scholar
1999-2002 National Research Service
Award, Public Health Service, NIH
1998 Excellence in
Graduate Research Award, Stony Brook University
1997 Excellence in
Research Award, Sigma Xi
1997 Travel Fellowship,
ASBMB
1994 Honors
in Teaching, Stony Brook University
1993 General Fellowship,
NCI, NIH
1992-1993 Student Research Training
Program Fellowship, NCI, NIH
PROFESSIONAL
SERVICE
2013 ad hoc Reviewer, National Science Centre, Poland
2008-2015 ad hoc Reviewer, Netherlands
Organization for Scientific Research
2007-2015 ad hoc Reviewer, National Science Foundation
2004-2015 ad hoc Reviewer, Nature, Molecular Cell,
PLoS ONE, Molecular and Cellular Biology, EMBO
Journal, Journal of Cell Biology,
RNA, Molecular Biology of the Cell, Cancer
Research, Journal of Visualized
Experiments, Insect Biochemistry and
Molecular Biology, and Acta
Biochimica et Biophysica Sinica
ADMINISTRATIVE SERVICE
2008-2015 Steering Committee, Program
in Cell Biology
2007, 2012 Krampitz Award Committee, Department
of Molecular Biology and Microbiology
2006,
2012 Retreat
Planning Committee, Department of Molecular Biology and Microbiology
2004,
2005 Faculty Advisor,
Biomedical Graduate Student Symposium
2003-2009 Committee on Appointments,
Promotions, and Tenure, Department of Molecular Biology and Microbiology
TEACHING
EXPERIENCE
Fall 2005-2015 Medical School Medium Size Group
Sessions
2004-2015 MBIO 435: Seminar in Molecular Biology/Microbiology
Fall 2010 BIOL 491:
Contemporary Biology And Biotechnology (guest lecture)
Fall
2009-2010 CLBY 526: Cell
Biology and Human Disease
Spring
2006 CLBY 422: Topics in
Cell Biology
Spring
2004-2006 MBIO/BIOC 620:
Transcription and Gene Regulation
Spring
2005 MBIO/BIOC 522:
Protein Phosphorylation and Cell Regulation
Fall 2004 GENE 515: Nuclear Structure
and Function
Spring 2001 BIOMG690:The Nucleus (guest
lecture); Cornell University
Spring 1994 BIO322: Animal Embryology; Stony
Brook University
Fall 1993 BIO150: Undergraduate
Biochemistry Laboratory; Stony Brook University
ADVISORY ACTIVITIES
2005-2007 M.S. advisor for Amy Graham
2005-2010 Ph.D. advisor for Megan
Smolko (née Mamolen)
2003-2009 Ph.D. advisor for Daniel Kiss
2003-2011 Undergraduate advisor; Connie
Martin, Alexandra Smith, Stephanie Davis, Whitney Huang, Lauren Dehan
1998-2002 Undergraduate research
advisor, Cornell University; Michael Seidman, Andy Eisberg, Karen Chinchilla
1995-1998 Undergraduate Research Mentor,
Stony Brook University
PRIOR RESEARCH
SUPPORT
1R01GM072820-01
(PI: Andrulis) 10/1/06-9/30/11 ($190,000 direct/yr/5
yrs.)
National
Institutes of General Medical Sciences
Compartmentalized
exosome structure and function
AFAR AA05105 (PI: Andrulis) 7/1/05-6/30/07 ($60,000)
American
Federation for Aging Research
Mechanisms of mRNA
Turnover during Aging
IRG-91-002-09
(PI: Andrulis) 1/01/04-12/31/04
($20,000)
American
Cancer Society
Specificity and
targeting of the exosome, a co-transcriptional mRNA surveillance complex, to
the BRCA1 gene
PUBLICATIONS
1.
Kleff, S., Andrulis, E. D., Anderson, C. W., and R. Sternglanz. (1995)
Identification of a Gene Encoding a Yeast Histone H4 Acetyltransferase. Journal
of Biological Chemistry, 270: 24672-24677.
2. Andrulis,
E. D., Neiman, A. M., Zappulla, D. C., and R. Sternglanz. (1998) Perinuclear
Localization of Chromatin Facilitates Transcriptional Silencing. Nature, 394: 592-595. (Erratum: Nature
395: 525; Reviewed in Curr. Opin. Genet. Dev. 1999; 9:199-205.)
3.
Matern, H. T., Yang, X., Andrulis, E., Sternglanz, R., Trepte, H.-H.,
and D. Gallwitz (2000) A Novel and Essential Golgi Membrane Protein Acts in or
Close to Transport Vesicle Docking. EMBO
Journal, 19:4485-4492.
4. Andrulis, E. D., Guzmán, E.,
Döring, P., Werner, J., and J. T. Lis (2000) High Resolution Localization of Drosophila Spt5 and Spt6 at Heat Shock
Genes in vivo: Roles in
Promoter-proximal Pausing and Transcription Elongation. Genes and Development, 14:
2635-2649.
5. Andrulis, E. D., Zappulla, D.
C., Ansari, A., Laiosa, C. V., Perrod, S., Gartenberg, M. R., and R. Sternglanz
(2002) ESC1, a Nuclear Periphery Protein Required for SIR4-based Plasmid
Partitioning and Anchoring. Molecular and
Cellular Biology, 22: 8292-8301.
6.
Andrulis, E. D., Werner, J., Erdjument-Bromage, H., Nazarian, A.,
Tempst, P., and J. T. Lis (2002) The RNA Processing Exosome is Linked to
Elongating RNA Polymerase II in Drosophila.
Nature, 420: 837-841. (Reviewed
in Nat. Struc. Bio. 2003; 10: 10-12.)
7. Saunders, A., Werner, J., Andrulis, E. D., Nakayama, T., Hirose, S., Reinberg, D. and J.T. Lis (2003) Tracking FACT and the RNA Polymerase II Elongation Complex Through Chromatin in vivo. Science, 301: 1094-1096. (Cover of Science; Perspective in Science, 301: 1053-1054)
8. Andrulis, E. D., Zappulla, D.
C., Alexieva-Botcheva, K., Evangelista, C., and R. Sternglanz (2004) One-hybrid
Screens at the Saccharomyces cerevisiae
HMR Locus Identify Novel
Transcriptional Silencing Factors. Genetics,
166: 631-635.
9.
Adelman, K., Marr, M.T., Werner, J., Saunders, A., Ni, Z., Andrulis, E.D.,
and J.T. Lis (2005) Efficient Release Through
the Promoter-proximal Stall Sites Requires Transcript Cleavage Factor
TFIIS. Molecular Cell 17:103-112.
10.
Graham, A. C., Kiss, D. L., and E. D. Andrulis (2006) Differential Distribution
of Exosome Subunits at the Nuclear Lamina and in Cytoplasmic Foci. Molecular Biology of the Cell, 17: 1399-1409.
11. Hattier, T., Andrulis, E. D., and A.
Tartakoff (2007) Immobility, Inheritance and Plasticity of Shape of the Yeast Nucleus.
BMC Cell Biology, 8: 47-64
12. Graham, A.C., Davis, S. M.[1],
and E. D. Andrulis (2009) Interdependence of Nucleocytoplasmic Distribution
and Interactions of Dis3 with Rrp6, the Core Exosome, and Importin-alpha3. Traffic 10: 499-513
13. Graham, A. C., Kiss, D. L., and E. D. Andrulis
(2009) Core Exosome-independent roles for Drosophila
Rrp6 in Cell Cycle Progression. Molecular Biology of the Cell, 20: 2242-2253.
14. Mamolen, M. and E.
D. Andrulis (2009) Characterization of the Drosophila melanogaster Dis3 Ribonuclease. Biochemical and Biophysical Research Communications, 390: 529-34.
15. Kiss, D.L. and E. D. Andrulis (2010)
Genome-wide Analysis Reveals Distinct Substrate Specificities of Rrp6, Dis3,
and Core Exosome Subunits. RNA, 16(4):781-91.
16.
Mamolen, M., Smith, A.1, and E. D. Andrulis (2010) Drosophila melanogaster Dis3 N-terminal Domains
are Required for Ribonuclease Activities, Nuclear Localization, and Exosome Interactions.
Nucleic Acids Research, 38(16):5507-17.
17. Kiss, D.L. and E. D. Andrulis. (2011) The Exozyme Model: A Continuum of Functionally Distinct Complexes.
RNA, 17(1):1-13.
18. Smith, S. B., Kiss, D. L., Turk,
E., Tartakoff, A. M., and E. D. Andrulis. (2011) Pronounced and Extensive Microtubule Defects
in a Saccharomyces cerevisiae DIS3 Mutant.
Yeast, 28:755-769.
19. Andrulis, E.D. (2011) Theory of the Origin, Evolution,
and Nature of Life. Life, 2:1-105. (Commentary by Publisher: Life, 2: 213-214)
20. Kiss, D.L., Hou, D., Gross, R.H., and E. D. Andrulis (2012) Dis3- and Exosome Subunit-responsive 3' mRNA Instability Elements.
Biochemical and Biophysical Research Communications, 423:461-466.
21. Hou, D., Ruiz, M., and E. D. Andrulis (2012) The Ribonuclease
Dis3 is an Essential Regulator of the Developmental Transcriptome. BMC Genomics, 13:359-370.
22. Turk, E., Das, V., Seibert, R[2]. and E.
D. Andrulis (2013) The Mitochondrial
RNA Landscape of Saccharomyces cerevisiae. PLoS
ONE, 8(10):
e78105.
SKILLS
•
Tissue cell culture growth, transfection, and analysis; yeast and bacterial
cell growth and transformation
•
Microscopy (electron, confocal, deconvolution, wide-field)
•
Fluorescence-based cell and organelle analysis (fluorophores, XFP fusions,
immunochemistry, FRAP, FRIP)
•
Cell-based and in vitro drug and aptamer screening, selection,
optimization, and analysis
•
Cell synchronization and sorting; cell and organelle fractionation and
extraction
•
Chromosome visualization (polytenes, karyotyping, mitotic staging,
lacO/LacI-GFP)
•
Mouse maintenance, sacrifice, and tumor cell injection, growth, and extraction
•
Yeast, bacterial, fly protein production, purification; genetic engineering
(knockout, RNAi, shRNA, P-element)
•
Viral infection, isolation, and cloning
•
Antibody production, purification, and characterization
•
Protein characterization (PAGE, western, IP, pull downs, ELISA, kinetics,
assays, TLC, EMSA, SPR)
•
In vitro transcription, translation, and chromatin assembly
•
Structure- and sequence-directed protein engineering
•
Molecular biological techniques (in silico, in vitro, and in
vivo cloning; PCR, site-directed mutagenesis)
•
Design, construction, and implementation of gene and protein expression vectors
•
Gene expression analysis (northern, RT-PCR, quantitative real-time RT-PCR,
run-on, ChIP)
•
Large-scale genomic, transcriptomic approaches (microarray, DNA-seq, ChIP-chip,
ChIP-seq, RNA-seq)
•
Genetic screens (one-, two-, and three-hybrid; suppressor; synthetic lethal,
conditional)
•
Bioinformatic packages and programs (BLAST, Galaxy, Avadis NGS, FASTQ, etc.)
•
AKTA platform and numerous HPLC strategies (affinity, IEC, SEC)
• Microsoft Office, Adobe Photoshop, GraphPad
PLATFORM
PRESENTATIONS
2013 Department of
Molecular Biology and Microbiology, CWRU School of Medicine
2012 Physis: From
Elementary Particles to Human Nature, University of the Basque Country
2011 Eukaryotic mRNA
Processing Meeting, Cold Spring Harbor Laboratory (CSH)
2011 Department of
Biochemistry, Emory University
2011 Department of
Biological Sciences, Cleveland State University (CSU)
2010 Center for RNA
Molecular Biology, CWRU School of Medicine
2009 Department of
Genetics, CWRU School of Medicine
2009 Department of Molecular
Biology and Microbiology, CWRU School of Medicine
2009 Department of Cell
Biology, Lerner Research Institute
2007 Department of
Biochemistry, CWRU School of Medicine
2006 Program in Cell
Biology Retreat, Squire Vallevue Farm
2005 Center for RNA Molecular
Biology, Case School of Medicine
2005 Department of
Pathology, CWRU School of Medicine
2004 Department of
Biological, Geological, and Environmental Sciences, CSU
2003 Department of
Molecular Biology and Microbiology, CWRU School of Medicine
2003 Department of
Oncological Sciences, Huntsman Cancer Institute
2003 Department of
Molecular Genetics, Ohio State University
2003 Center for RNA
Molecular Biology, CWRU School of Medicine
2003 Department of
Biological Sciences, SUNY at Albany
2003 Department of Cell
Biology, Albert Einstein College of Medicine
2003 Norris Comprehensive
Cancer Center, University of Southern California
2003 Department of
Biology, University of Rochester
2002 Department of
Biological Sciences, SUNY at Buffalo
2002 Department of Cell
and Developmental Biology, Vanderbilt University
2002 Program in Gene
Function and Expression, University of Massachusetts Medical Center
2002 Gordon Research
Conference (GRC): Nucleic Acids, Roger Williams University
1998 Institute for Cell
and Developmental Biology, Stony Brook University
1996 Mid-Atlantic Yeast
Meeting, University of Maryland Baltimore County
MEETINGS
AND POSTER PRESENTATIONS
2012 Astrobiology Science
Conference, Atlanta, GA
2012 GRC: Origin of Life,
Galveston, TX
2011 GRC: Geobiology,
Ventura Beach, CA
2010 75th
Symposium: Nuclear Structure and Function, CSH
2009 RNA Conference,
University of Wisconsin, Madison
2007 RNA Conference,
University of Wisconsin, Madison
2006 American Federation
for Aging Research (AFAR) conference, Santa Barbara, CA
2006 Dynamic Organization
of Nuclear Function, CSH
2004 GRC: The Biology of
Post-transcriptional Gene Regulation, Proctor Academy, NH
2001 Mechanisms of
Eukaryotic Transcription, CSH
2000 GRC: Chromatin
Structure and Function, Tilton Academy, NH
1996 GRC: Nuclear
Proteins, Chromatin Structure and Gene Regulation, Tilton Academy, NH
POLICY
AND PLANNING EXPERIENCE
2008-2015 Steering
Committee, Program in Cell Biology
2007, 2012
Lester O. Krampitz Award
Committee
2006, 2012 Retreat Planning Committee,
Department of Molecular Biology & Microbiology (MBM)
2010, 2011 Medical
Thesis Review Committee
2004, 2005 Faculty
Advisor, Biomedical Graduate Student Symposium
2003-2009 Committee on Appointments,
Promotions, and Tenure, MBM
2000-2002 Postdoctoral Organization,
Cornell University
CWRU
AFFILIATIONS AND MEETING GROUPS
2006-2015 Member,
Comprehensive Cancer Center
2005-2015 Member, Program in Cell
Biology
2004-2015 Member, Drosophila Group Meeting (Andrulis, Broihier, Harte, Mizutani, Salz
labs)
2004-2015 Member, Program in
Developmental Biology
2004-2015 Mentor, Developmental
Biologist Training Program
2003-2015 Mentor, Biomedical Scientist
Training Program
2003-2015 Mentor, Cell and Molecular
Biology Training Program
2005-2008 Adjunct
Member, Center for RNA Molecular Biology
2004-2007 Member, RNA Genetics Group
Meeting (Andrulis, Luo, Matera, Salz, Wise labs)
CWRU THESIS COMMITTEES
2003-2012 Chairman; Student, Julian
Wong; Advisor, Jonathan Karn, Ph.D., MBM
2006-2010 Advisor; Student, Megan
Mamolen, MBM
2006-2010 Chairman; Student, Nicole
Dietenberger; Advisor, Patrick Viollier, Ph.D., MBM
2004-2009 Member; Student, Matthew
Johnson; Advisor, Helen Salz, Ph.D., Department of Genetics
2003-2009 Advisor; Student, Daniel
Kiss, MBM
2006-2008 Chairman; Student, Julie
Jadlowsky; Advisor, Koh Fujinaga, Ph.D., MBM
2003-2008 Chairman; Student, Felipe
Bendezu; Advisor, Piet de Boer, Ph.D., MBM
2006-2008 Advisor and Member; Student,
Amy Graham; Department of Biology
2006-2007 Chairman; Student, Jonathan
Bell; Advisor, Catherine Patterson, Ph.D., MBM
2004-2005 Member; Student, Thomas
Hattier; Advisor, Alan Tartakoff, Ph.D., Department of Pathology
REFERENCES
Catherine
Patterson, Ph.D.,
Director of Scientific Research, Pharmaceutical Development Group, St.
Petersburg, FL; 216-526-5928; cepatt123@yahoo.com
Peter
Harte, Ph.D.,
Professor, Department of Genetics, CWRU School of Medicine, Cleveland, OH;
(216) 368-6417; peter.harte@case.edu
Alan M. Tartakoff, Ph.D., Professor, Department
of Pathology, Program in Cell Biology, CWRU School of Medicine, Cleveland, OH;
(216) 368-5544; amt10@case.edu
